Application

Anti-Nestin, clone 8B8, ZooMAb is a recombinant Rabbit Monoclonal antibody that detects Nestin and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Nestin in SH-SY5Y cells.Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Nestin in lysate from Human H9 Stem Cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

GST/His-tagged recombinant fragment corresponding to 150 amino acids from the C-terminal region of human Nestin.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 8B8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Nestin. It targets an epitope with in 150 amino acids from the C-terminal region.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Nestin (UniProt: P48681) is encoded by the NES (also known as Nbla00170) gene (Gene ID: 10763) in human. Nestin is a class VI intermediate filament protein that is required for survival, renewal, and mitogen-stimulated proliferation of neural progenitor cells. Nestin expression is up-regulated in most mitotic cells and is downregulated upon differentiation. Upon terminal neural differentiation Nestin levels are shown to be down-regulated and replaced by neurofilaments. Nestin is also shown to be expressed in kidney in a developmentally regulated pattern. Its expression is also associated with tubulointerstitial injury and can serve as a marker for peritubular endothelial cell injury and tubulointerstitial fibrosis. Nestin is reported to be constitutively phosphorylated and its phosphorylation state increases during mitosis when the cytoplasmic intermediate filament network is reorganized. Nestin has a short N-terminus and an unusually long C-terminus region that results in complex binding to an array of structural proteins. The short N-terminus leads to preferential intermediate filaments formation with heterodimers and heterotetramers and the long C-terminal portion functions as links or bridges between intermediate filaments and microtubules. Nestin can exist in homodimeric or homotetrameric form, however in mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, it can form heterodimers, which can assemble to form intermediate filaments. Nestin is shown to promote the disassembly of phosphorylated vimentin intermediate filaments during mitosis and play a role in the trafficking and distribution of intermediate filament proteins to daughter cells during progenitor cell division. (Ref.: Xie, L., et al. (2015). Stem Cells Int. 2015:762098; Tomioka, M., et al. (2010). 15(5); 568-574).